Hinweis zum Urheberrecht
Bericht / Forschungsbericht / Abhandlung zugänglich unter
URL: https://epub.sub.uni-hamburg.de/epub/volltexte/2015/45607/
Development and application of rapid methods for quantification and cultivation of methanogens in bio-methane producing fermentors
Kim, Yong Sung
| SWD-Schlagwörter: |
| Biogas , Biodiversität , Fluoreszenzmikroskop , Intensität , Fermentation , Hochschulschrift |
| Freie Schlagwörter (Englisch): |
| Indicator , Quantification , Classification , Morphology , Isolation |
| BK - Klassifikation: |
| 58.30 |
| DDC-Sachgruppe: |
| Naturwissenschaften |
| Dokumentart: |
| Bericht / Forschungsbericht / Abhandlung |
| Sprache: |
| Englisch |
| Erstellungsjahr: |
| 2015 |
| Publikationsdatum: |
| 01.10.2015 |
| Kurzfassung auf Englisch: |
| This thesis describes the development of simple methods for isolation of
methanogens as well as a method for microscopic quantification. Using an
extensive literature review, a detailed account of the method development
including modification, improvement, and validation of the technique was
described. Additionally, a new indicator was consequently developed using
the microscopic technique as a rapid tool for assessment of anaerobic
digesters. This indicator was empirically established, based on complex
interaction between methanogenic cell counts, chemical parameters and
biodiversity. The indicator, so-called quantitative microscopic
fingerprinting index (QMFI), was the first approach to combine microscopic
cell counts with environmental factors including volatile fatty acids and
biodiversity to find and objectify methanogenic activity in anaerobic
digesters. Using the microscopic technique, it was found that
morphological changes of methanogens could be an early and new indicator
regarding temperature and ammonia perturbation as a new parameter for the
monitoring of anaerobic digesters. Additionally, a simple cultivation and
isolation method for strict anaerobic methanogens without a cost-intensive
anaerobic chamber was developed. An anaerobic petri-dish box was developed
to enable simple selection of methanogenic colonies on a single petri-dish
under pressurised conditions with H2-CO2 as sole energy and carbon source.
In this study, several methanogens were isolated from anaerobic digesters
by the developed method. The development of both microscopic
quantification and isolation techniques could contribute to the analysis
of methanogens in anaerobic digesters as a new simple, rapid, and reliable
tool to increase the process efficiency of digesters.
|
